English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/182822
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Magnetic nanoparticles covalently immobilized on cell surfaces for localized magnetic hyperthermia

AuthorsIdiago López, Francisco Javier; Moreno Antolín, Eduardo; Fratila, Raluca M.; Fuente, Jesús M. de la
Issue Date2018
CitationI Jornada de Jóvenes Investigadores de Cerámica y Vidrio en el ICMA (2018)
AbstractHerein we report the use of biorthogonal click chemistry as a tool to immobilize magnetic nanoparticles (MNPs) and to localize magnetic hyperthermia on living cell membranes. The incorporation of azide reporters on cell membranes based on metabolic glycoengineering allows the “click” attachment of cyclooctyne-functionalized MNPs on cell membranes through strain-promoted [3+2] azide-alkyne cycloaddition (SPAAC). Hydrophobic 12 nm iron oxide MNPs were synthesized following by thermal decomposition and transferred to water by coating with a fluorescent amphiphilic polymer – poly (maleic anhydride-alt-1-octadecene), PMAO-TAMRA. The MNPs were further functionalized step-wise with polyethyleneglycol (PEG) to increase colloidal stability and with a strained alkyne at different percentages of the total reactive groups of the MNPs. Gel electrophoresis, thermogravimetric analysis and ζ-potential measurements confirmed the successful functionalization of the MNPs. In addition, confocal microscopy results revealed an effective covalent immobilization of MNPs on MCF-7 cell membranes at a 50% strained alkyne-functionalization. We are currently investigating the MH behaviour of the immobilized MNPs.
DescriptionResumen del trabajo presentado a la I Jornada de jóvenes investigadores de cerámica y vidrio en el ICMA, celebrada el 20 de marzo de 2018 en la Universidad de Zaragoza.
URIhttp://hdl.handle.net/10261/182822
Appears in Collections:(ICMA) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.