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dc.contributor.authorRomero-Fernández, Mariaes_ES
dc.contributor.authorMoreno-Pérez, Soniaes_ES
dc.contributor.authorOrrego, Alejandro H.es_ES
dc.contributor.authorMartins de Oliveira, Sandroes_ES
dc.contributor.authorSantamaría, Ramón I.es_ES
dc.contributor.authorDíaz, Margaritaes_ES
dc.contributor.authorGuisán, José Manueles_ES
dc.contributor.authorRocha-Martín, Javieres_ES
dc.date.accessioned2019-05-30T07:11:18Z-
dc.date.available2019-05-30T07:11:18Z-
dc.date.issued2018-10-
dc.identifier.citationBioresource Technology 266: 249-258 (2018)es_ES
dc.identifier.issn0960-8524-
dc.identifier.urihttp://hdl.handle.net/10261/182816-
dc.description.abstractThe present study focuses on the development and optimization of a packed-bed reactor (PBR) for continuous production of xylooligosaccharides (XOS) from xylan. For this purpose, three different methacrylic polymer-based supports (Relizyme R403/S, Purolite P8204F and Purolite P8215F) activated with glyoxyl groups were morphologically characterized and screened for the multipoint covalent immobilization of a xylanase. Based on its physical and mechanical properties, maximum protein loading and thermal stability, Relizyme R403/S was selected to set up a PRB for continuous production of XOS from corncob xylan. The specific productivity for XOS at 10 mL/min flow rate was 3277 gXOS genzyme−1 h−1 with a PBR. This PBR conserved >90% of its initial activity after 120 h of continuous operation.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (projects BIO2012-36861 and CTQ2015- 70348). The authors would like to thank Spanish Ministry of Economy, Industry and Competitiveness for financial support awarded the first author (BES-2013-065396). Javier Rocha-Martin is grateful for the “Juan de la Cierva” fellowship (IJCI-2014-19260) funded by the Spanish Ministry of Economy, Industry and Competitiveness.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/CTQ2015-70348es_ES
dc.relationMINECO/ICTI2013-2016/IJCI-2014-19260es_ES
dc.rightsclosedAccesses_ES
dc.subjectXylan hydrolysises_ES
dc.subjectXylooligosaccharideses_ES
dc.subjectEnzyme immobilizationes_ES
dc.subjectBioprocess intensificationes_ES
dc.subjectFlow reactores_ES
dc.titleDesigning continuous flow reaction of xylan hydrolysis for xylooligosaccharides production in packed-bed reactors using xylanase immobilized on methacrylic polymer-based supportses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.biortech.2018.06.070-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.biortech.2018.06.070es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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