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Título: | High throughput sequencing analysis of Trypanosoma brucei DRBD3/PTB1-bound mRNAs |
Autor: | Das, Anish; Bellofatto, Vivian; Rosenfeld, Jeffrey; Carrington, Mark; Romero-Zaliz, Rocio; del Val, Coral; Estévez, Antonio M. CSIC ORCID | Palabras clave: | mRNA translation RNA-binding proteins Trypanosoma brucei |
Fecha de publicación: | ene-2015 | Editor: | Elsevier | Citación: | Molecular and Biochemical Parasitology | Resumen: | Trypanosomes are early-branched eukaryotes that show an unusual dependence on post-transcriptional mechanisms to regulate gene expression. RNA-binding proteins are crucial in controlling mRNA fate in these organisms, but their RNA substrates remain largely unknown. Here we have analyzed on a global scale the mRNAs associated with the Trypanosoma brucei RNA-binding protein DRBD3/PTB1, by capturing ribonucleoprotein complexes using UV cross-linking and subsequent immunoprecipitation. DRBD3/PTB1 associates with many transcripts encoding ribosomal proteins and translation factors. Consequently, silencing of DRBD3/PTB1 expression altered the protein synthesis rate. DRBD3/PTB1 also binds to mRNAs encoding the enzymes required to obtain energy through the oxidation of proline to succinate. We hypothesize that DRBD3/PTB1 is a key player in RNA regulon-based gene control influencing protein synthesis in trypanosomes. | Descripción: | (C) 2015 Elsevier B.V. All rights reserved. | Versión del editor: | http://dx.doi.org/10.1016/j.molbiopara.2015.02.003 | URI: | http://hdl.handle.net/10261/182646 | DOI: | 10.1016/j.molbiopara.2015.02.003 | ISSN: | 0166-6851 | E-ISSN: | 1872-9428 |
Aparece en las colecciones: | (IPBLN) Artículos |
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