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Title

Optimization of the immobilization of sweet potato amylase using glutaraldehyde-agarose support. Characterization of the immobilized enzyme

AuthorsTavano, Olga Luisa; Fernández-Lafuente, Roberto ; Goulart, Antonio José; Monti, Rubens
KeywordsGlutaraldehyde
Beta-amylase
Sweet potato
Agarose
Immobilization
Issue DateJul-2013
PublisherElsevier
CitationProcess Biochemistry 48(7): 1054-1058 (2013)
AbstractA simplified procedure for the preparation of immobilized beta-amylase using non-purified extract from fresh sweet potato tubers is established in this paper, using differently activated agarose supports. Beta-amylase glutaraldehyde derivative was the preparation with best features, presenting improved temperature and pH stability and activity. The possibility of reusing the amylase was also shown, when this immobilized enzyme was fully active for five cycles of use. However, immobilization decreased enzyme activity to around 15%. This seems to be mainly due to diffusion limitations of the starch inside the pores of the biocatalyst particles. A fifteen-fold increase in the Km was noticed, while the decrease of Vmax was only 30% (10.1 U mg−1 protein and 7.03 U mg−1 protein for free and immobilized preparations, respectively).
Publisher version (URL)https://doi.org/10.1016/j.procbio.2013.05.009
URIhttp://hdl.handle.net/10261/182559
DOI10.1016/j.procbio.2013.05.009
ISSN1359-5113
E-ISSN1873-3298
Appears in Collections:(ICP) Artículos
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