English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/18203
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Cell cycle and cell mortality of Alexandrium minutum (Dinophyceae) under small-scale turbulence conditions

AuthorsLlaveria, Gisela ; Figueroa, Rosa Isabel ; Garcés, Esther ; Berdalet, Elisa
KeywordsAlexandrium minutum
Cell cycle
Coulter counter
Flow cytometry
Small-scale turbulence
Issue DateSep-2009
PublisherBlackwell Publishing
CitationJournal of Phycology 45(5): 1106-1115 (2009)
AbstractDecreased net population growth rates and cellular abundances have been observed in dinoflagellate species exposed to small-scale turbulence. Here, we investigated whether these effects were caused by alterations in the cell cycle and/or by cell mortality and, in turn, whether these two mechanisms depended on the duration of exposure to turbulence. The study was conducted on the toxic dinoflagellate Alexandrium minutum Halim, with the same experimental design and setup used in previous studies to allow direct comparison among results. A combination of microscopy and Coulter Counter measurements allowed us to detect cell mortality, based on the biovolume of broken cells and thecae. The turbulence applied during the exponential growth phase caused an immediate transitory arrest in the G2/M phase, but significant mortality did not occur. This finding suggests that high intensities of small-scale turbulence can alter the cell division, likely affecting the correct chromosome segregation during the dinomitosis. When shaking persisted for >4 d, mortality signals and presence of anomalously swollen cells appeared, hinting at the activation of mechanisms that induce programmed cell death. Our study suggests that the sensitivity of dinoflagellates to turbulence may drive these organisms to find the most favorable (calm) conditions to complete their division cycle.
Description10 pages, 5 figures, 1 table
Publisher version (URL)https://doi.org/10.1111/j.1529-8817.2009.00740.x
Appears in Collections:(ICM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.