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dc.contributor.authorUrrutia, P.-
dc.contributor.authorRodríguez-Colinas, Bárbara-
dc.contributor.authorFernández Arrojo, Lucía-
dc.contributor.authorBallesteros Olmo, Antonio-
dc.contributor.authorWilson, L.-
dc.contributor.authorIllanes, Andrés-
dc.contributor.authorPlou Gasca, Francisco José-
dc.date.accessioned2019-05-17T12:12:08Z-
dc.date.available2019-05-17T12:12:08Z-
dc.date.issued2013-
dc.identifier.citationJournal of Agricultural and Food Chemistry 61(5): 1081-1087 (2013)-
dc.identifier.issn0021-8561-
dc.identifier.urihttp://hdl.handle.net/10261/181661-
dc.description.abstractThe synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product was 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) of total GOS. In contrast with previous reports, the presence of at least five disaccharides was detected, which accounted for 40% of the total GOS at the point of maximum GOS concentration (allolactose and 6-galactobiose were the major products). A. oryzae β-galactosidase showed a preference to form β(1→6) bonds, followed by β(1→3) and β(1→4) linkages. Results were compared with those obtained with β-galactosidases from Kluyveromyces lactis and Bacillus circulans. The highest GOS yield and specific productivity were achieved with B. circulans β-galactosidase. The specificity of the linkages formed and distribution of di-, tri-, and higher GOS varied significantly among the three β-galactosidases. © 2013 American Chemical Society.-
dc.publisherAmerican Chemical Society-
dc.rightsclosedAccess-
dc.subjectPrebiotics-
dc.subjectOligosaccharides-
dc.subjectTransglycosylation-
dc.subjectβ-galactosidase-
dc.subjectGlycosidase-
dc.subjectGalactooligosaccharides-
dc.titleDetailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae-
dc.typeartículo-
dc.identifier.doi10.1021/jf304354u-
dc.relation.publisherversionhttps://doi.org/10.1021/jf304354u-
dc.identifier.e-issn1520-5118-
dc.date.updated2019-05-17T12:12:09Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.relation.csic-
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