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Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae

AuthorsUrrutia, P.; Rodríguez-Colinas, Bárbara ; Fernández Arrojo, Lucía ; Ballesteros Olmo, Antonio ; Wilson, L.; Illanes, Andrés; Plou Gasca, Francisco José
Issue Date2013
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 61(5): 1081-1087 (2013)
AbstractThe synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum GOS yield, measured by HPAEC-PAD, was 26.8% w/w of total carbohydrates, obtained at approximately 70% lactose conversion. No less than 17 carbohydrates were identified; the major transgalactosylation product was 6′-O-β-galactosyl-lactose, representing nearly one-third (in weight) of total GOS. In contrast with previous reports, the presence of at least five disaccharides was detected, which accounted for 40% of the total GOS at the point of maximum GOS concentration (allolactose and 6-galactobiose were the major products). A. oryzae β-galactosidase showed a preference to form β(1→6) bonds, followed by β(1→3) and β(1→4) linkages. Results were compared with those obtained with β-galactosidases from Kluyveromyces lactis and Bacillus circulans. The highest GOS yield and specific productivity were achieved with B. circulans β-galactosidase. The specificity of the linkages formed and distribution of di-, tri-, and higher GOS varied significantly among the three β-galactosidases. © 2013 American Chemical Society.
Publisher version (URL)https://doi.org/10.1021/jf304354u
Appears in Collections:(ICP) Artículos
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