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Closed Access item Xanthone production in calli and suspended cells of Hypericum perforatum

Authors:Dias, Alberto C. P.
Seabra, Rosa M.
Andrade, Paula B.
Ferreres, Federico
Fernandes-Ferreira, Manuel
Keywords:Calli, Cell suspensions, Hypericaceae, Hypericum perforatum, Phytohormones, Xanthones
Issue Date:2001
Citation:Journal of Plant Physiology 158(7): 821-827 (2001)
Abstract:Calli and suspended cells of Hypericum perforatum accumulated xanthones with the 1,3,6,7 and 1,3,5,6-oxygenation pattern. Xanthone accumulation was highly influenced by hormone supplementation. Calli grown with 4.5 μmol/L 2,4-D + 2.3 μmol/L KIN had the highest specific xanthone production observed (0.73 percnt; DW). The use of BA instead of KIN reduced xanthone accumulation, resulting in the lowest production achieved (0.24 percnt; DW). This effect was attenuated when both hormones were present. Calli displayed a positive linear relationship between NAA concentration, in the range of 4.5-22.5 μmol/L, and total xanthone accumulation and the xanthone 1,3,6,7/1,3,5,6 ratio. However, the production of 1,3,5,6 xanthones was similar in all NAA concentrations studied.
Description:7 pages, 4 figures, 1 table.
Publisher version (URL):http://dx.doi.org/10.1078/0176-1617-00195
Appears in Collections:(CEBAS) Artículos

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