English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/181501
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Ultrasensitive detection of HIV-1 p24 antigen by a hybrid nanomechanical-optoplasmonic platform with potential for detecting HIV-1 at first week after infection

AuthorsKosaka, Priscila M. ; Pini, Valerio ; Calleja, Montserrat ; Tamayo de Miguel, Francisco Javier
Issue Date15-Feb-2017
PublisherPublic Library of Science
CitationPLoS ONE 12(2): e0171899 (2017)
AbstractEarly detection of HIV infection is the best way to prevent spread of the disease and to improve the efficiency of the antiretroviral therapy. Nucleic acid amplification tests (NAAT) have become the gold-standard for detecting low-concentrations of the virus in blood. However, these methods are technically demanding and cost-prohibitive in developing countries. Immunoassays are more affordable and can be more easily adapted for point-of-care diagnosis. However, the sensitivity so far of these methods has been too low. We here report the development of a sandwich immunoassay that combines nanomechanical and optoplasmonic transduction methods for detecting the HIV-1 capsid antigen p24 in human serum. The immunoreactions take place on the surface of a compliant microcantilever where gold nanoparticles are used as both mechanical and plasmonic labels. The microcantilever acts as both a mechanical resonator and an optical cavity for the transduction of the mechanical and plasmonic signals. The limit of detection of the immunoassay is 10−17 g/mL that is equivalent to one virion in 10 mL of plasma. This is 5 orders of magnitude better than last generation of approved immunoassays and 2 orders of magnitude better than NAAT. This technology meets the demands to be produced en masse at low cost and the capability for miniaturization to be used at the point-of-care.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0171899
URIhttp://hdl.handle.net/10261/181501
DOI10.1371/journal.pone.0171899
E-ISSN1932-6203
Appears in Collections:(IMN-CNM) Artículos
Files in This Item:
File Description SizeFormat 
Ultrasensitive detection_Kosaka.pdf2,03 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.