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Título: | Qualitative and quantitative analysis of endophyte alkaloids in perennial ryegrass using Near-Infrared Spectroscopy |
Autor: | Soto Barajas, Milton Carlos CSIC ORCID; Zabalgogeazcoa, Iñigo ; Vázquez de Aldana, Beatriz R. CSIC ORCID ; González, Inmaculada CSIC | Palabras clave: | Ergovaline Epichloë endophytes Grassland Lolium perenne Lolitrem B 22 peramine |
Fecha de publicación: | 2017 | Editor: | John Wiley & Sons | Citación: | Journal of the Science of Food and Agriculture 97(14): 5028-5036 (2017) | Resumen: | [BACKGROUND]
Near-infrared reflectance spectroscopy (NIRS) has been widely used in forage quality control because it is faster, cleaner and less expensive than conventional chemical procedures. In Lolium perenne (perennial ryegrass), one of the most important forage grasses, the infection by asymptomatic Epichloë fungal endophytes alters the plant nutritional quality due to the production of alkaloids. In this research, we developed a rapid method based on NIRS to detect and quantify endophyte alkaloids (peramine, lolitrem B and ergovaline) using a heterogeneous set of L. perenne plants obtained from wild grasslands and cultivars. [RESULTS] NIR spectra from dried grass samples were recorded and classified according to the absence or presence of alkaloids, based on reference methods. The best discriminant equations for detection of alkaloids classified correctly 94.4%, 87.5% and 92.9% of plants containing peramine, lolitrem B and ergovaline, respectively. The quantitative NIR equations obtained by modified partial least squares (MPLS) algorithm had coefficients of correlation of 0.93, 0.41, and 0.76 for peramine, lolitrem B and ergovaline respectively. [CONCLUSION] NIRS is a suitable tool for qualitative analysis of endophyte alkaloids in grasses and for the accurate quantification of peramine and ergovaline. |
Descripción: | 33 páginas, 4 figuras y 3 tablas. | Versión del editor: | https://doi.org/10.1002/jsfa.8383 | URI: | http://hdl.handle.net/10261/181254 | DOI: | 10.1002/jsfa.8383 | ISSN: | 0022-5142 | E-ISSN: | 1097-0010 |
Aparece en las colecciones: | (IRNASA) Artículos |
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Qualitative and quantitative analysis of endophyte alkaloids in perennial.pdf | "Artículo principal" | 407,12 kB | Adobe PDF | Visualizar/Abrir |
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