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dc.contributor.authorEncinar, Marioes_ES
dc.contributor.authorCasado, Santiagoes_ES
dc.contributor.authorCalzado-Martín, Aliciaes_ES
dc.contributor.authorNatale, P.es_ES
dc.contributor.authorSan Paulo, Álvaroes_ES
dc.contributor.authorCalleja, Montserrates_ES
dc.contributor.authorVélez, Mariselaes_ES
dc.contributor.authorMonroy, Franciscoes_ES
dc.contributor.authorLópez-Montero, Ivánes_ES
dc.date.accessioned2019-05-09T12:29:16Z-
dc.date.available2019-05-09T12:29:16Z-
dc.date.issued2017-01-01-
dc.identifier.citationColloids and Surfaces - B - Biointerfaces 149: 174-183 (2017)es_ES
dc.identifier.issn0927-7765-
dc.identifier.urihttp://hdl.handle.net/10261/181170-
dc.description.abstractErythrocyte membranes have been particularly useful as a model for studies of membrane structure and mechanics. Native erythroid membranes can be electroformed as giant unilamellar vesicles (eGUVs). In the presence of ATP, the erythroid membrane proteins of eGUVs rearrange into protein networks at the microscale. Here, we present a detailed nanomechanical study of individual protein microfilaments forming the protein networks of eGUVs when spread on supporting surfaces. Using Peak Force tapping Atomic Force Microscopy (PF-AFM) in liquid environment we have obtained the mechanical maps of the composite lipid-protein networks supported on solid surface. In the absence of ATP, the protein pool was characterized by a Young’s Modulus Epool ≈ 5–15 MPa whereas the complex filaments were found softer after protein supramolecular rearrangement; Efil ≈ 0.4 MPa. The observed protein softening and reassembling could be relevant for understanding the mechanisms of cytoskeleton reorganization found in pathological erythrocytes or erythrocytes that are affected by biological agents.es_ES
dc.description.sponsorshipThis work was supported by the ERC Starting Grant “mitochon” (ERC-StG-2013-883188) and “Programa Ramon y Cajal” (RYC-2013-12609) from the Spanish Ministry of Economy MINECO (I. L-M.), the ERC Starting Grant “NANOFORCELLS” (ERC-StG-2011-278860) (M. E., A. C-M., M. C.), the project FORCE-for-FUTURE (CSD2010-00024) (A. S. P.) and FIS2012-35723 from MINECO (F.M.).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/RYC-2013-12609es_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/278860es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectGiant vesicleses_ES
dc.subjectErythrocytees_ES
dc.subjectLipid membranees_ES
dc.subjectCytoskeleton reconstitutiones_ES
dc.subjectAFMes_ES
dc.subjectPeakForce tapping quantitative nanomechanical mappinges_ES
dc.titleNanomechanical properties of composite protein networks of erythroid membranes at lipid surfaceses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.colsurfb.2016.10.022-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.colsurfb.2016.10.022es_ES
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000781es_ES
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