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Direct detection of OXA-48 carbapenemase gene in lysate samples through changes in mechanical properties of DNA monolayers upon hybridization

AuthorsDomínguez, Carmen M.; Ramos Vega, Daniel ; Mingorance, Jesús; Fierro, José L.G.; Tamayo de Miguel, Francisco Javier ; Calleja, Montserrat
Issue Date2-Jan-2018
PublisherAmerican Chemical Society
CitationAnalytical Chemistry - Columbus 90(1): 968–973 (2018)
AbstractCarbapenem-resistant Enterobacteriaceae have recently become an important cause of morbidity and mortality due to healthcare-associated infections. Most commonly used diagnostic methods are incompatible with fast and accurate directed therapy. We report here the direct identification of the blaOXA48 gene, which codes for the carbapenemase OXA-48, in lysate samples from Klebsiella pneumoniae. The method is PCR-free and label-free. It is based on the measurement of changes in the stiffness of DNA self-assembled monolayers anchored to microcantilevers that occur as a consequence of the hybridization. The stiffness of the DNA layer is measured through changes of the sensor resonance frequency upon hybridization and at varying relative humidity.
Publisher version (URL)http://dx.doi.org/10.1021/acs.analchem.7b04094
Appears in Collections:(IMN-CNM) Artículos
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