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The Transcriptome of Verticillium dahliae Responds Differentially Depending on the Disease Susceptibility Level of the Olive (Olea europaea L.) Cultivar

AuthorsJiménez-Ruiz, Jaime; Leyva Pérez, M. de la O ; Gómez-Lama Cabanás, Carmen ; Barroso-Albarracín, Juan Bautista ; Luque, Francisco; Mercado-Blanco, Jesús
KeywordsDefoliating pathotype
Vascular pathogen
Verticillium dahliae transcriptome
Verticillium wilt of olive
Issue Date27-Mar-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationGenes 10(4): 251 (2019)
AbstractAmong biotic constraints affecting olive trees cultivation worldwide, the soil-borne fungus <i>Verticillium dahliae</i> is considered one of the most serious threats. Olive cultivars display differential susceptibility to the disease, but our knowledge on the pathogen’s responses when infecting varieties differing in susceptibility is scarce. A comparative transcriptomic analysis (RNA-seq) was conducted in olive cultivars Picual (susceptible) and Frantoio (tolerant). RNA samples originated from roots during the first two weeks after inoculation with <i>V. dahliae</i> defoliating (D) pathotype. <i>Verticillium dahliae</i> mRNA amount was overwhelmingly higher in roots of the susceptible cultivar, indicating that proliferation of pathogen biomass is favored in ‘Picual’. A significant larger number of <i>V. dahliae</i> unigenes (11 fold) were only induced in this cultivar. Seven clusters of differentially expressed genes (DEG) were identified according to time-course expression patterns. Unigenes potentially coding for niche-adaptation, pathogenicity, virulence and microsclerotia development were induced in ‘Picual’, while in ‘Frantoio’ expression remained negligible or null. <i>Verticillium dahliae</i> D pathotype transcriptome responses are qualitatively and quantitatively different, and depend on cultivar susceptibility level. The much larger <i>V. dahliae</i> biomass found in ‘Picual’ roots is a consequence of both host and pathogen DEG explaining, to a large extent, the higher aggressiveness exerted over this cultivar.
Publisher version (URL)http://dx.doi.org/10.3390/genes10040251
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