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The helicase PIF1 facilitates resection over sequences prone to forming G4 structures

AuthorsJimeno, Sonia ; Camarillo, Rosa; Mejías-Navarro, Fernando; Fernández-Ávila, María Jesús ; Soria-Bretones, Isabel; Prados-Carvajal, Rosario; Huertas Sánchez, Pablo
KeywordsDNA end resection
DNA recombination
DSB repair
DNA repair
Issue Date18-Sep-2018
CitationCell Reports 24(12): 3262-3273.e4 (2018)
AbstractDNA breaks are complex lesions that can be repaired either by non-homologous end joining (NHEJ) or by homologous recombination (HR). The decision between these two routes of DNA repair is a key point of the DNA damage response (DDR) that is controlled by DNA resection. The core machinery catalyzing the resection process is well established. However, little is known about the additional requirements of DNA resection over DNA structures with high complexity. Here, we found evidence that the human helicase PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes. Indeed, PIF1 is recruited to the site of DNA damage and physically interacts with proteins involved in DNA resection, and its depletion causes DNA damage sensitivity and a reduction of HR efficiency. Moreover, G4 stabilization by itself hampers DNA resection, a phenomenon suppressed by PIF1 overexpression
Publisher version (URL)https://doi.org/10.1016/j.celrep.2018.08.047
Appears in Collections:(CABIMER) Artículos
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