English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/180170
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A novel redox-sensing histidine kinase that controls carbon catabolite repression in Azoarcus sp. CIB

AuthorsValderrama, J. Andrés ; Gómez-Álvarez, Helena ; Martín-Moldes, Zaira ; Berbís, Manuel Álvaro ; Cañada, F. Javier ; Durante-Rodríguez, Gonzalo ; Díaz, Eduardo
KeywordsCatabolite repression
Quinones
Redox switch
Sensor kinase
Issue Date9-Apr-2019
PublisherAmerican Society for Microbiology
CitationmBio 10:e00059-19 (2019)
AbstractWe have identified and characterized the AccS multidomain sensor kinase that mediates the activation of the AccR master regulator involved in carbon catabolite repression (CCR) of the anaerobic catabolism of aromatic compounds in Azoarcus sp. CIB. A truncated AccS protein that contains only the soluble C-terminal autokinase module (AccS′) accounts for the succinate-dependent CCR control. In vitro assays with purified AccS′ revealed its autophosphorylation, phosphotransfer from AccS′∼P to the Asp60 residue of AccR, and the phosphatase activity toward its phosphorylated response regulator, indicating that the equilibrium between the kinase and phosphatase activities of AccS′ may control the phosphorylation state of the AccR transcriptional regulator. Oxidized quinones, e.g., ubiquinone 0 and menadione, switched the AccS′ autokinase activity off, and three conserved Cys residues, which are not essential for catalysis, are involved in such inhibition. Thiol oxidation by quinones caused a change in the oligomeric state of the AccS′ dimer resulting in the formation of an inactive monomer. This thiol-based redox switch is tuned by the cellular energy state, which can change depending on the carbon source that the cells are using. This work expands the functional diversity of redox-sensitive sensor kinases, showing that they can control new bacterial processes such as CCR of the anaerobic catabolism of aromatic compounds. The AccSR two-component system is conserved in the genomes of some betaproteobacteria, where it might play a more general role in controlling the global metabolic state according to carbon availability.
Description16 p.-8 fig.-1 tab.
Publisher version (URL)https://doi.org/10.1128/mBio.00059-19
URIhttp://hdl.handle.net/10261/180170
DOI10.1128/mBio.00059-19
E-ISSN2150-7511
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
mBio_Valderrama_2019.pdfArtículo principal931,43 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.