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Título: | Flexibility of the jaw-lobe region in RNA polymerase I influences transcriptional elongation |
Autor: | Darrière, Tommy; Pilsl, Michael; Sarthou, Marie-Kerguelen; Chauvier, Adrien; Genty, Titouan; Audibert, Sylvain; Dez, Christophe; Leger-Silvestre, Isabelle; Normand, Christophe; Kwapisz, Marta; Calvo, Olga CSIC ORCID; Fernández-Tornero, Carlos CSIC ORCID ; Tschochner, Herbert; Gadal, Olivier | Fecha de publicación: | 2018 | Citación: | III Meeting Red de Excelencia Temática (2018) | Resumen: | In growing eukaryotic cells, most transcriptional activity involves synthesis of ribosomal RNA (rRNA) by RNA polymerase I (Pol I). Yeast Pol I is composed by 14 subunits with an overall mass of 600 kDa. Deletion of Pol I subunit A49, which plays a role both in transcription initiation and elongation, causes a severe growth defect. Genetic analysis allowed us to isolate a set of mutants on different Pol I subunits that suppress the A49 deletion phenotype. Most suppressor mutants cluster at the Pol I jaw-lobe region, which encompasses the jaw in subunit A190, the lobe in subunit A135, and the N-terminal and liker domains of subunit A12. We use computational analysis of available Pol I structures to propose a role for the jaw-lobe region in the transition between transcription initiation and elongation. Finally, we show that the most efficient suppressor mutant exhibits increased rRNA production both in vivo and in vitro. | Descripción: | Resumen del trabajo presentado al III Meeting Red de Excelencia Temática: "RNA Life", celebrado en Salamanca del 24 al 26 de septiembre de 2018. | URI: | http://hdl.handle.net/10261/180057 |
Aparece en las colecciones: | (IBFG) Comunicaciones congresos (CIB) Comunicaciones congresos |
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