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Title

Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing

AuthorsRuiz, Elizabeth; Ramírez, César A.; Casas, Julián Camilo; Ospina, María I.; Requena, José María ; Puerta, Concepción J.
KeywordsRegulationofgeneexpression
RNAbinding proteins [RBP]
Untranslatedregion [UTR]
Trypanosomacruzi
LYT1 gene
Issue Date25-Sep-2018
PublisherPontificia Universidad Javeriana
CitationUniversitas Scientiarum 23: 267- 290 (2018)
AbstractIn trypanosomatids,geneexpressionismainlyregulated at posttranscriptional level,throughmechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of Them RNAs,whichaltogetherformribonucleoproteiccomplexes [RNP] that define thefateofthemRNA.Thepre-mRNAderivedfromthe LYT1 gene of Trypanosomacruzi, isprocessedbyalternative trans-splicing, resultingin differentmRNAswhichcodefortheisoformsmLYT1andkLYT1,proteins having differential expression,cellular location and function.The aim of this study was to characterizethe5¿and3¿UTRsofthe LYT1 mRNAsasthe initial steptowardstheobjectiveofidentificationoftheRBPsresponsiblefor theirdifferentialexpression.Thepresenceofthetwotypesof5¿UTRswere confirmedintwo T.cruzi isolatesbelongingtotheDTUI,thus,corroborating theoccurrenceofalternative trans-splicing alsointhe LYT1 geneofthis T.cruzi DTU.Inaddition,forthefirsttime,wasunscoveredtheexistenceoftwo types of LYT1 mRNAstranscripts,differinginlengthby116nts,thatare generatedbyalternativepolyadenylation.Furthermore,an in-silico analysis of theexperimentallyobtainedUTRs,andtenadditional LYT1 sequences retrievedfromTritrypDBandGenBankdatabases,togetherwithathoroughly searchofstructuralmotifs,showedaremarkableconservationofrelevant structuralmotifspreviouslyassociatedwithRNAmetabolisminthedifferent UTRs; theseelementsmightbeinvolvedinthedifferentialstage
URIhttp://hdl.handle.net/10261/178774
Identifiersdoi: 10.11144/Javeriana.SC23-2.cotm
issn: 0122-7483
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