English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/178345
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Antiproliferative activity of abietane diterpenoids against human tumor cells

AuthorsBurmistrova, Olga; Simões, M. Fátima; Rijo, Patricia; Quintana, José; Bermejo, Jaime ; Estévez, Francisco
Issue Date18-Jul-2013
PublisherAmerican Chemical Society
CitationJournal of Natural Products 76: 1413-1423 (2013)
AbstractIn the present study, the cytotoxicity of 30 diterpenoids with an abietane or a halimane skeleton was determined against five human tumor cell lines (HL-60, U937, Molt-3, SK-MEL-1, and MCF-7). Diterpenoids containing an abietane skeleton including taxodone (1) and taxodione (2), as well as the semisynthetic derivatives 12, 14, 15, 17, and 22, were the most cytotoxic compounds for human leukemia cells. Overexpression of the protective mitochondrial proteins Bcl-2 and Bcl-xL did not confer resistance to abietane diterpene-induced cytotoxicity. Studies performed on HL-60 cells indicated that growth inhibition triggered by compounds 1, 12, 14, and 15 was caused by induction of apoptosis. This was prevented by the nonspecific caspase inhibitor Z-VAD-FMK and, in the case of compounds 14 and 15, reduced by the selective caspase-8 inhibitor Z-IETD-FMK. Cell death induced by these abietane diterpenes was found to be associated with the release of mitochondrial proteins, including cytochrome c, Smac/DIABLO, and AIF (apoptosis-inducing factor), accompanied by dissipation of the mitochondrial membrane potential (ΔΨ), and modulated by inhibition of extracellular signal-regulated kinases signaling and the p38 mitogen-activated protein kinase pathway.
URIhttp://hdl.handle.net/10261/178345
Identifiersdoi: 10.1021/np400172k
issn: 0163-3864
e-issn: 1520-6025
Appears in Collections:(IPNA) Artículos
Files in This Item:
File Description SizeFormat 
Antiproliferative_Burmistrova_JNP.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.