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Title

Optogenetic navigation of routes leading to protein amyloidogenesis in bacteria

AuthorsGiraldo, R.
KeywordsLOV2
Optobiotics
Phase transitions
Prion-like
RepA-WH1
Issue Date15-Mar-2019
PublisherElsevier
CitationJournal of Molecular Biology 431 (6) 1186-1202 (2019)
AbstractModulation of liquid–liquid and liquid–hydrogel phase transitions is central to avoid the cytotoxic aggregation of proteins in eukaryotic cells, but knowledge on its relevance in bacteria is limited. Here the power of optogenetics to engineer proteins as light-responsive switches has been used to control the balance between solubility and aggregation for LOV2–WH1, a chimera between the plant blue light-responsive domain LOV2 and the bacterial prion-like protein RepA-WH1. These proteins were first linked by fusing, as a continuous α-helix, the C-terminal photo-transducer Jα helix in LOV2 with the N-terminal domain-closure α1 helix in RepA-WH1, and then improved for light-responsiveness by including mutations in the Jα moiety. In the darkness and in a crowded solution in vitro, LOV2–WH1 nucleates the irreversible assembly of amyloid fibers into a hydrogel. However, under blue light illumination, LOV2–WH1 assembles as soluble oligomers. When expressed in Escherichia coli, LOV2–WH1 forms in the darkness large intracellular amyloid inclusions compatible with bacterial proliferation. Strikingly, under blue light, LOV2–WH1 aggregates decrease in size, while they become detrimental for bacterial growth. LOV2–WH1 optogenetics governs the assembly of mutually exclusive inert amyloid fibers or cytotoxic oligomers, thus enabling the navigation of the conformational landscape of protein amyloidogenesis to generate potential photo-activated anti-bacterial devices (optobiotics).
Description32 p.-1 graph. abst.-7 fig.-8 fig.supl.
Publisher version (URL)https://doi.org/10.1016/j.jmb.2019.01.037
URIhttp://hdl.handle.net/10261/178100
DOI10.1016/j.jmb.2019.01.037
ISSN0022-2836
E-ISSN1089-8638
Appears in Collections:(CIB) Artículos
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