English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/177961
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Functional characterization and structural modelling of Helianthus annuus (sunflower) ketoacyl-CoA synthases and their role in seed oil composition

AuthorsGonzález-Mellado, Damián ; Salas, Joaquín J. ; Venegas-Calerón, Mónica ; Moreno-Pérez, Antonio J.; Garcés Mancheño, Rafael ; Martínez-Force, Enrique
KeywordsHelianthus annuus
Very long chain fatty acids
Fatty acid elongase
Ketoacyl-CoA synthase
Sunflower seeds
Issue Date7-Mar-2019
PublisherSpringer
CitationPlanta: (2019)
AbstractMain conclusion The enzymes HaKCS1 and HaKCS2 are expressed in sunflower seeds and contribute to elongation of C18 fatty acids, resulting in the C20–C24 fatty acids in sunflower oil. Most plant fatty acids are produced by plastidial soluble fatty acid synthases that produce fatty acids of up to 18 carbon atoms. However, further acyl chain elongations can take place in the endoplasmic reticulum, catalysed by membrane-bound synthases that act on acyl-CoAs. The condensing enzymes of these complexes are the ketoacyl-CoA synthase (KCSs), responsible for the synthesis of very long chain fatty acids (VLCFAs) and their derivatives in plants, these including waxes and cuticle hydrocarbons, as well as fatty aldehydes. Sunflower seeds accumulate oil that contains around 2–3% of VLCFAs and studies of the fatty acid elongase activity in developing sunflower embryos indicate that two different KCS isoforms drive the synthesis of these fatty acids. Here, two cDNAs encoding distinct KCSs were amplified from RNAs extracted from developing sunflower embryos and named HaKCS1 and HaKCS2. These genes are expressed in developing seeds during the period of oil accumulation and they are clear candidates to condition sunflower oil synthesis. These two KCS cDNAs complement a yeast elongase null mutant and when expressed in yeast, they alter the host’s fatty acid profile, proving the encoded KCSs are functional. The structure of these enzymes was modelled and their contribution to the presence of VLCFAs in sunflower oil is discussed based on the results obtained.
Description39 Páginas.-- 8 Figuras
Publisher version (URL)http://dx.doi.org/10.1007/s00425-019-03126-1
URIhttp://hdl.handle.net/10261/177961
ISSN0032-0935
E-ISSN1432-2048
Appears in Collections:(IG) Artículos
Files in This Item:
File Description SizeFormat 
PostP_2019_Planta_Functional.pdf Embargoed until March 7, 2020Artículo principal855,85 kBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.