English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/177650
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Production of xylo-oligosaccharides (XOS) by controlled hydrolysis of xylan using immobilized xylanase from Aspergillus niger with improved properties

AuthorsAragon, Caio C.; Ruiz-Matute, Ana I. ; Corzo, Nieves ; Monti, Rubens; Guisán, José Manuel ; Mateo, César
KeywordsXOS production
Enzyme thermostabilization
Biocatalysis
Aspergillus niger
Xylanase
Issue Date2018
CitationIntegrative Food, Nutrition and Metabolism 5(4): 1-9 (2018)
AbstractThe production of xylo-oligosaccharides from xylan using an optimal immobilized catalyst of xylanase from Aspergillus niger is presented. The enzyme extract has several xylanases with different properties doing necessary the development of cheap and simple methods to purify them at industrial scale. The enzyme was successfully purified, immobilized and highly stabilized using a simple protocol. The principal purified xylanase was a 34 KDa protein corresponding with 50% of the endo-xylanase activity of the total strain. Among the different immobilization assayed protocols, the use of aldehyde support allowed the complete immobilization of this fraction keeping 80% of its initial catalytic activity. An optimization of this method promoted a stabilization factor of around 1100-fold more stable than soluble enzyme. The use of the optimal catalyst allowed a maximum hydrolysis degree of 73.4%. The optimization of the reaction conditions (different time and temperature) allowed producing 62% (11.93 mg/mL) of interesting xylooligo-saccharides (XOS2-XOS6) (Figure 1).
Publisher version (URL)http://dx.doi.org/10.15761/IFNM.1000225
URIhttp://hdl.handle.net/10261/177650
Identifiersdoi: 10.15761/IFNM.1000225
issn: 2056-8339
Appears in Collections:(CIAL) Artículos
(IQOG) Artículos
Files in This Item:
File Description SizeFormat 
Integr Food Nutr Metab, 2018.pdf1,56 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.