English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/177334
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

ZipN is an essential FtsZ membrane tether and contributes to the septal localization of SepJ in the flamentous cyanobacterium Anabaena

AuthorsCamargo, Sergio ; Picossi, Silvia ; Corrales-Guerrero, Laura ; Valladares, Ana ; Arévalo, Sergio; Herrera, Antonia
Issue Date2019
PublisherNature Publishing Group
CitationScientific Reports, ) 9:2744 (2019)
AbstractThe organismic unit of heterocyst-forming cyanobacteria is a flament of communicating cells connected by septal junctions, proteinaceous structures bridging the cytoplasms of contiguous cells. This distinct bacterial organization is preserved during cell division. In Anabaena, deletion of the zipN gene could not be segregated. We generated strain CSL109 that expresses zipN from a synthetic regulatable promoter. Under conditions of ZipN depletion, cells progressively enlarged, refecting restricted cell division, and showed drastic morphological alterations including cell detachment from the flaments, to fnish lysing. In contrast to the wild-type localization in midcell Z-rings, FtsZ was found in delocalized aggregates in strain CSL109. Consistently, the proportion of membrane-associated to soluble FtsZ in fractionated cell extracts was lower in CSL109. Bacterial two-hybrid analysis showed that ZipN interacts with FtsZ and other cell-division proteins including cytoplasmic Ftn6 and SepF, and polytopic FtsW, FtsX, FtsQ and FtsI. Additionally, ZipN interacted with the septal protein SepJ, and in CSL109 depletion of ZipN was concomitant with a progressive loss of septal specifcity of SepJ. Thus, in Anabaena ZipN represents an essential FtsZ membrane tether and an organizer of the divisome, and it contributes to the conformation of septal structures for flament integrity and intercellular communication.
Publisher version (URL)https://doi.org/10.1038/s41598-019-39336-6
URIhttp://hdl.handle.net/10261/177334
DOI10.1038/s41598-019-39336-6
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
s41598-019-39336-6.pdf3,69 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.