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http://hdl.handle.net/10261/177294
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Serafín, Verónica | es_ES |
dc.contributor.author | Torrente-Rodríguez, Rebeca M. | es_ES |
dc.contributor.author | Batlle, Montserrat | es_ES |
dc.contributor.author | García de Frutos, Pablo | es_ES |
dc.contributor.author | Campuzano, Susana | es_ES |
dc.contributor.author | Yáñez-Sedeño, Pilar | es_ES |
dc.contributor.author | Pingarrón, José Manuel | es_ES |
dc.date.accessioned | 2019-03-05T13:34:21Z | - |
dc.date.available | 2019-03-05T13:34:21Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | Sensors and Actuators - B - Chemical Biochemical Sensors 240: 1251-1256 (2017) | es_ES |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10261/177294 | - |
dc.description.abstract | A sensitive and rapid method for the determination of the clinically relevant biomarker receptor tyrosine kinase AXL in serum involving amperometric disposable immunosensors is reported. The target protein was sandwiched between a specific capture antibody covalently immobilized on screen-printed carbon electrodes modified with electropolymerized poly(pyrrolepropionic acid) and a biotinylated detector antibody labeled with a streptavidin-horseradish peroxidase conjugate. The amperometric responses were measured at −0.20 V vs the Ag pseudo-reference electrode of the SPCE upon the addition of H2O2 in the presence of hydroquinone (HQ) as mediator. This integrated immunosensing platform showed a low limit of detection (337 pg mL−1), a good selectivity against other non-target serum proteins, and provided results statistically in agreement with those obtained by using a commercial ELISA kit. These attractive features together with the simplicity and easy automation and miniaturization of the required instrumentation make the developed methodology a promising alternative in the development of devices for on-site clinical analysis. | es_ES |
dc.description.sponsorship | The financial support of projects: Retos Colaboración RTC2015-4184-1 (cofinanced by the Ministry of Economy and Competitivity and FEDER), CTQ2015-70023-R and CTQ2015- 64402-C2-1-R (Spanish Ministry of Economy and Competitivity Research Projects) and S2013/MT-3029 (NANOAVANSENS Program from the Comunidad de Madrid) are gratefully acknowledged. R.M. Torrente-Rodríguez acknowledges a predoctoral contract from the Spanish Ministerio de Economía y Competitividad. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC2015-4184-1 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70023-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015- 64402-C2-1-R | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Receptor tyrosine kinase AXL | es_ES |
dc.subject | Electrochemical immunosensor | es_ES |
dc.subject | Human serumHeart failure | es_ES |
dc.subject | Cancer | es_ES |
dc.title | Electrochemical immunosensor for receptor tyrosine kinase AXL using poly(pyrrolepropionic acid)-modified disposable electrodes | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.snb.2016.09.109 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.snb.2016.09.109 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
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