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dc.contributor.authorSanz, Pascuales_ES
dc.contributor.authorViana, Rosaes_ES
dc.contributor.authorGarcía-Gimeno, María Adelaidaes_ES
dc.date.accessioned2019-03-04T12:37:25Z-
dc.date.available2019-03-04T12:37:25Z-
dc.date.issued2018-04-12-
dc.identifier.citationMethods in Molecular Biology 1732:143-157 (2018)es_ES
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10261/177178-
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-7598-3_9 (15 páginas, 5 figuras)es_ES
dc.description.abstractMammalian AMP-activated protein kinase (AMPK) is a Ser/Thr protein kinase that acts as a crucial energy sensor in the cell. Since AMPK plays a key role in a multitude of different pathways in the cell, major efforts have been concentrated to elucidate its signaling network, mainly by the identification of AMPK downstream targets. In this chapter we describe a yeast two-hybrid method for the direct evaluation of the interaction between an AMPK subunit and putative substrates.es_ES
dc.description.sponsorshipThis study was supported by grants from the Spanish Ministry of Education and Science (SAF2014-54604-C3-1-R) and Generalitat Valenciana (PrometeoII/2014/029) to P.S.es_ES
dc.language.isoenges_ES
dc.publisherHumana Presses_ES
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-54604-C3-1-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectAMPKes_ES
dc.subjectBait plasmides_ES
dc.subjectPrey plasmides_ES
dc.subjectβ-Galactosidasees_ES
dc.subjectYeast two-hybrides_ES
dc.titleAMPK Protein Interaction Analyses by Yeast Two-Hybrides_ES
dc.typeartículoes_ES
dc.identifier.doi10.1007/978-1-4939-7598-3_9-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/978-1-4939-7598-3_9es_ES
dc.identifier.e-issn1940-6029-
dc.embargo.terms2019-04-12es_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)es_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.contributor.orcidSanz, Pascual [0000-0002-2399-4103]es_ES
dc.contributor.orcidViana, Rosa [0000-0002-0036-0669]es_ES
dc.contributor.orcidGarcia-Gimeno, Maria Adelaida [0000-0002-6483-3912]es_ES
dc.identifier.pmid29480473-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.openairetypeartículo-
item.languageiso639-1en-
item.grantfulltextopen-
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