English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/177178
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


AMPK Protein Interaction Analyses by Yeast Two-Hybrid

AuthorsSanz, Pascual ; Viana, Rosa ; Garcia-Gimeno, Maria Adelaida
Bait plasmid
Prey plasmid
Yeast two-hybrid
Issue Date12-Apr-2018
PublisherHumana Press
CitationMethods in Molecular Biology 1732:143-157 (2018)
AbstractMammalian AMP-activated protein kinase (AMPK) is a Ser/Thr protein kinase that acts as a crucial energy sensor in the cell. Since AMPK plays a key role in a multitude of different pathways in the cell, major efforts have been concentrated to elucidate its signaling network, mainly by the identification of AMPK downstream targets. In this chapter we describe a yeast two-hybrid method for the direct evaluation of the interaction between an AMPK subunit and putative substrates.
DescriptionThis is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-7598-3_9 (15 páginas, 5 figuras)
Publisher version (URL)http://dx.doi.org/10.1007/978-1-4939-7598-3_9
Appears in Collections:(IBV) Artículos
Files in This Item:
File Description SizeFormat 
Author copy MiMB 2018.pdf691,47 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.