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Targeting of prolamin by rnai in bread wheat

AuthorsBarro Losada, Francisco ; Gil Humanes, Javier
Issue Date22-Dec-2016
CitationWO2016202805 A2
AbstractThe present disclosure concerns the specific silencing of at least one of, but preferable all, of α (alpha), β (beta), γ (gamma), and ω (omega) gliadins and the LMW (low molecular weight) glutenin subunits of hard wheat and flour by interference RNA (iRNA) through use of a polynucleotide that is transcribed into an hpRNA (hairpin RNA). Furthermore, the present disclosure also concerns a vector, cell, plant, or seed comprising the polynucleotide. Expression can be specifically directed to particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of the gene that codes for a D-hordein.
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