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Registro de acceso cerrado Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars

Autor :Pereira, José Alberto
Oliveira, Ivo
Sousa, Anabela
Valentão, Patricia
Andrade, Paula B.
Ferreira, Isabel C. F. R.
Ferreres, Federico
Bento, Albino
Seabra, Rosa M.
Estevinho, Letícia
Palabras clave :Walnut leaves, Phenolics, Antimicrobial activity, Antioxidant properties
Fecha de publicación :nov-2007
Editor:Elsevier
Citación :Food and Chemical Toxicology 45(11): 2287-2295 (2007)
Resumen:Different cultivars of walnut (Juglans regia L.) leaves (Cv. Lara, Franquette, Mayette, Marbot, Mellanaise and Parisienne) grown in Portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. Phenolics analysis was performed by reversed-phase HPLC/DAD and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, quercetin 3-arabinoside, quercetin 3-xyloside and quercetin 3-rhamnoside. The antimicrobial capacity was screened against Gram positive (Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans). Walnut leaves selectively inhibited the growth of Gram positive bacteria, being B. cereus the most susceptible one (MIC 0.1 mg/mL). Gram negative bacteria and fungi were resistant to the extracts at 100 mg/mL. Lara walnut leaves were also submitted to antibacterial assays using 18 clinical isolates of Staphylococcus sp. Antioxidant activity was accessed by the reducing power assay, the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and β-carotene linoleate model system. In a general way, all of the studied walnut leaves cultivars presented high antioxidant activity (EC50 values lower than 1 mg/mL), being Cv. Lara the most effective one.
Descripción :9 páginas, 6 figuras, 4 tablas.-- El PDF es la copia de autor.
Versión del editor:http://dx.doi.org/10.1016/j.fct.2007.06.004
URI :http://hdl.handle.net/10261/17680
ISSN:0278-6915
DOI:10.1016/j.fct.2007.06.004
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