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Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs

AuthorsDomínguez, Juan; Acosta, Fermín; Pérez-Lago, Laura; Sambrano, Dilcia; Batista, Victoria; De La Guardia, Carolina; Abascal, Estefanía; Chiner-Oms, Álvaro ; Comas, Iñaki ; González, Prudencio; Bravo, Jaime; Del Cid, Pedro; Rosas, Samantha; Muñoz, Patricia; Goodridge, Amador; García de Viedma, Darío
Strain-specific PCR
Tuberculosis and other mycobacteria
Whole-genome sequencing
Issue DateMar-2019
PublisherCenters for Disease Control and Prevention (U.S.)
CitationEmerging Infectious Disease 25(3):507-514 (2019)
AbstractSystematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A-F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism-based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain-specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A-C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain-specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs.
Description8 páginas, 2 figuras, 1 tabla
Publisher version (URL)http://dx.doi.org/10.3201/eid2503.181593
Appears in Collections:(IBV) Artículos
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