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Título: | The relevance of the UPS in fatty liver graft preservation: A new approach for IGL-1 and HTK solutions |
Autor: | Panisello-Roselló, Arnau CSIC ORCID; Verde, Eva; Zaouali, Mohamed A. CSIC ORCID; Flores, Marta; Alva, Norma; López, Alexandre CSIC; Folch-Puy, Emma CSIC ORCID ; Carbonell, Teresa; Hotter, Georgina CSIC ORCID ; Adam, René; Roselló-Catafau, Joan CSIC ORCID | Palabras clave: | Ubiquitin proteasome system Cold ischemic injury Fatty liver preservation IGL-1 HTK ATP AMPK and nitric oxide |
Fecha de publicación: | 31-oct-2017 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | International Journal of Molecular Sciences 18(11): 2287 (2017) | Resumen: | The 26S proteasome is the central proteolytic machinery of the ubiquitin proteasome system (UPS), which is involved in the degradation of ubiquitinated protein substrates. Recently, UPS inhibition has been shown to be a key factor in fatty liver graft preservation during organ cold storage using University of Wisconsin solution (UW) and Institute Georges Lopez (IGL-1) solutions. However, the merits of IGL-1 and histidine-tryptophan-ketoglutarate (HTK) solutions for fatty liver preservation have not been compared. Fatty liver grafts from obese Zücker rats were preserved for 24 h at 4 ◦C. Aspartate aminotransferase and alanine aminotransferase (AST/ALT), glutamate dehydrogenase (GLDH), ATP, adenosine monophosphate protein kinase (AMPK), e-NOS, proteasome activity and liver polyubiquitinated proteins were determined. IGL-1 solution prevented ATP breakdown during cold-storage preservation of steatotic livers to a greater extent than HTK solution. There were concomitant increases in AMPK activation, e-NOS (endothelial NOS (NO synthase)) expression and UPS inhibition. UPS activity is closely related to the composition of the solution used to preserve the organ. IGL-1 solution provided significantly better protection against ischemia-reperfusion for cold-stored fatty liver grafts than HTK solution. The effect is exerted through the activation of the protective AMPK signaling pathway, an increase in e-NOS expression and a dysregulation of the UPS. | Versión del editor: | https://doi.org/10.3390/ijms18112287 | URI: | http://hdl.handle.net/10261/176579 | DOI: | 10.3390/ijms18112287 | ISSN: | 1661-6596 | E-ISSN: | 1422-0067 |
Aparece en las colecciones: | (IIBB) Artículos |
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Revelance_Panisello.pdf | 2,72 MB | Adobe PDF | Visualizar/Abrir |
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