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Título: | I-TRAQ proteomic approach to understand low concentration citrate capped gold nanoparticle stress in the marine bivalve Ruditapes philippinarum |
Autor: | Volland, Moritz CSIC; Torreblanca, Amparo; Gavidia Josa, Raquel; Blasco, Julián CSIC ORCID ; Hampel, Miriam CSIC ORCID | Fecha de publicación: | nov-2016 | Citación: | 6th Congress of the Spanish Proteomic Society (2016) | Resumen: | Over the last decades engineered gold nanoparticles (AuNPs) have been developed for and introduced into a growing number of commercial and industrial products. Up to date limited information is available on the ecotoxicological responses in non-target organisms, in par-ticular under environmental relevant concentrations. Notwithstanding their promising applica-tions, increased understanding of their behaviour and effects is required to manage potential risk for non-target organisms.We studied differential protein expression in response to citrate capped AuNPs (21.5 ± 2.9 nm) in the digestive gland of the bivalve Ruditapes philippinarum after 1 and 7 days of expo-sure at an environmental relevant concentration (750 ng L-1), using a 2nd-generation (iTRAQ-8plex) proteomic approach.2200 proteins were identified to be simultaneously expressed in control and treatment and a regularized regression approach (Elastic Net) identified 105 of those to be expressed dif-ferentially. Homology-based functional annotation could be successfully performed for 77% of all sequences and 75% of the differentially expressed sequences and subsequent analysis identified potential enrichment in various stress and injury related functions. Identified pro-teins may be useful as biomarkers of environmental nanoparticle pollution and provide insight about the mode of action of engineered gold nanoparticles. | Descripción: | Trabajo presentado en el 6th Congress of the Spanish Proteomic Society, celebrado en Cádiz del 15 al 18 de noviembre de 2016. | URI: | http://hdl.handle.net/10261/176423 |
Aparece en las colecciones: | (ICMAN) Comunicaciones congresos |
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