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Uptake, elimination and oxidative stress response to 0.75 ug/L citrate gold nanoparticle exposure in the marine clam E. philippinarum

AuthorsVolland, Moritz; Hampel, Miriam ; Trombini, Chiara ; Martos-Sitcha, Juan Antonio ; Martínez-Rodríguez, Gonzalo ; Blasco, Julián
Issue DateNov-2014
Citation2nd Marine NanoEcoSafety Workshop (2014)
AbstractGold nanopar ticles (AuNPs) h ave unique physical and chemical properties and are therefore used and developed for a wide r ange of novel applications in medicine, biology and chem istry. However in recent years concern has been raised over their ability to enter cells, organelles and n uclei, and provoke oxidative stress. In a laboratory-based experiment the non -t arget marine bivalve Ruditapes philippinarum was used as a model organismo Uptake, elimination and molec ular effects of ~20 nm citrate AuNPs at 0.75 Ilg 1-1 were studied over 7 days exposure and equal length elimination periodo Our results demonstrate that the AuNPs were readily taken up into the digestive glan d > gilIs. We further observed limited oxidative stress and inflammatory responses. The activity of several antioxidant en zy mes was increased in an organ sp ecific manner, with the digestive gland ex.hibiting the majority of its enzyme activity within 2,1 hours of exposure. Gene expression analysis highlighted the involvement of inflammatory re sponse genes in digestive gland tissue, as measured by changes in T NF-a and Gadd45 expression. Suggesting that one m echanism of citrate AuNP toxicity i5 well described by the oxidative stress par adigm. Further a significant elimination of Au from the digestive tract within t he purification period was observed, with excretion being an important pathway. In conclusiol1, wh ile not producing significant oxidative dam ag e, concern is rajsed regardi ng the effects of ch ronic AuNP exposure.
DescriptionTrabajo presentado en el 2nd Marine NanoEcoSafety Workshop (MANET), celebrado en Palermo el 17 y 18 de noviembre de 2014.
Appears in Collections:(ICMAN) Comunicaciones congresos
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