Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/17608
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Título : Esterases activities and lipid peroxidation levels in muscle tissue of the shanny Lipophrys pholis along several sites from the Portuguese Coast
Autor : Solé, Montserrat, Lobera, Gemma, Lima, Daniela, Reis-Henriques, Maria Armanda, Santos, Miguel Machado
Palabras clave : Lipophorys pholis
NW European marine ecosystems
Cholinesterases
Lipid peroxidation
Fecha de publicación : 25-Feb-2008
Editor: Elsevier
Citación : Marine Pollution Bulletin 56(5): 999-1007 (2008)
Resumen: This study is part of a project aiming to validate the use of the intertidal shanny Lipophorys pholis as a sentinel species in pollution monitoring in NW European marine ecosystems. To this end, a characterisation of acethylcholin (AChE), butyrylcholin (BChE) and propionylcholin (PrChE) esterases in L. pholis muscle was performed and the results indicated that AChE was predominant. Furthermore, the use of eserine sulphate and BW284c51 (0.64–800 μM), and iso-OMPA (0.08–16 mM), confirmed the measurement of true cholinesterases (ChEs) as well as the presence of pseudocholinesterases. The field application of these markers to L. pholis, sampled in seven locations along the Portuguese coast, revealed that fish were likely to be affected by neurotoxic compounds. This was indicated by the significant depletion of AChE (p < 0.05) in animals collected at urban and industrialised sites, compared with those from reference locations. The inclusion of a marker of effect, measured as lipid peroxidation levels in muscle tissue, also revealed the existence of site differences. Overall, the study further validates the utility of L. pholis in pollution monitoring studies.
Descripción : 9 pages, 2 figures, 4 tables.-- PMID: 18295805 [PubMed].-- Printed version published May 2008.
Versión del editor: http://dx.doi.org/10.1016/j.marpolbul.2008.01.016
URI : http://hdl.handle.net/10261/17608
ISSN: 0025-326X
DOI: 10.1016/j.marpolbul.2008.01.016
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