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Title

Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation

AuthorsHussain, Tanweer; Llácer, José Luis ; Wimberly, Brian T.; Kieft, Jeffrey S.; Ramakrishnan, V.
KeywordsIF1
IF2
IF3
cryo-EM
fMet-tRNA
Initiation
Ribosome
Start codon
Structures
Translation
Issue Date22-Sep-2016
PublisherElsevier
CitationCell 167(1):133-144.e13 (2016)
AbstractIn bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.
Description26 páginas, 7 figuras. Contiene Material suplementario
Publisher version (URL)http://dx.doi.org/10.1016/j.cell.2016.08.074
URIhttp://hdl.handle.net/10261/175906
DOI10.1016/j.cell.2016.08.074
ISSN0092-8674
E-ISSN1097-4172
Appears in Collections:(IBV) Artículos
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