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Cloning and expression of KIT ligand and their receptors in the European seabass

AutorSimón-Díaz, Marisol; Martos-Sitcha, Juan Antonio ; Mancera, Juan Miguel; Prat, Francisco
Fecha de publicaciónjul-2017
CitaciónXI Congreso de la Asociación Ibérica de Endocrinología Comparada (2017)
ResumenThe Kit system is receiving increasing attention in recent years. In mammals, among other functions, this system acts as regulator in several reproductive functions, especially during the early stages of gonadal development. The Kit system is integrated by Kit ligand (or Stem Cell Factor), which is an important growth factor, and its receptor Kit. In order to have a better knowledge on the control of early stages of gametogenesis in fish, the objective of this study is to know whether Kitlg/Kit system can also be involved in the regulation of the initial phases of gametogenesis in the European seabass Dicentrarchus labrax. Despite very little is known about the Kit system in fish, two forms of the kitlg (kitlga and kitlgb) and two forms of the receptor (kita and kitb) has been described in zebrafish. Similarly, in the European seabass, we have detected two forms of the kit receptor but only one form of the kitlg by blast analysis against the NCBI whole-genome shotgun contigs (wgs) database for D. labrax (sequences CBXY010000001 to CBXY010037781). The sequences encoding kitlg, kita and kitb were confirmed by PCR using ovarian and testis cDNA and specific primers designed to the respective genomic sequences. Resulting amplicons were sequenced showing open reading frames (ORFs) of 810 bp, 2973 bp and 2871 bp encoding 269, 990, 956 amino acids, respectively. Tissue specificity analysis by non-quantitative RT-PCR from adult European seabass samples revealed expression of these genes in almost all tissues studied including ovary and testis. These results together with the distribution and expression patterns, during gonadal development show evidences of an apparently higher expression in males than in females and in immature than mature gonads.
DescripciónTrabajo presentado en el XI Congreso de la Asociación Ibérica de Endocrinología Comparada, celebrado en Vigo del 13 al 15 de julio de 2017.
URIhttp://hdl.handle.net/10261/175795
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