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dc.contributor.authorAlcolea, Pedro J.es_ES
dc.contributor.authorAlonso, Anaes_ES
dc.contributor.authorBaugh, Lorenes_ES
dc.contributor.authorPaisie, Carolynes_ES
dc.contributor.authorRamasamy, G.es_ES
dc.contributor.authorSekar, Aarthies_ES
dc.contributor.authorSur, Aakashes_ES
dc.contributor.authorJiménez, Maribeles_ES
dc.contributor.authorMolina, Ricardoes_ES
dc.contributor.authorLarraga, Vicentees_ES
dc.contributor.authorMyler, P. J.es_ES
dc.identifier.citationParasitol Int 67(4):476-480 (2019)es_ES
dc.description16 p.-1 fig.-2 tab.- 3 ad. file.es_ES
dc.description.abstractLeishmania infantum is responsible for human and canine leishmaniasis in the Mediterranean basin, where the major vector is Phlebotomus perniciosus. Because isolation of sufficient parasites from the sand fly gut is technically challenging, axenic cultivation of promastigotes is routinely used to obtain material for biochemical and genetic analyses. Here, we report the use of Spliced Leader RNA-seq (SL-seq) to compare transcript abundance in cultured promastigotes and those obtained from the whole midgut of the sand fly 5 days after infection. SL-seq allows for amplification of RNA from the parasite avoiding contamination with RNA from the gut of the insect. The study has been performed by means of a single technical replicate comparing pools of samples obtained from sand fly-derived (sfPro) and axenic culture promastigotes (acPro). Although there was a moderate correlation (R2 = 0.83) in gene expression, 793 genes showed significantly different (≥2-fold, p <0.05) mRNA levels in sand fly-derived promastigotes and in culture, of which 31 were up-regulated ≥8-fold (p < 10-8 in most cases). These included several genes that are typically up-regulated during metacyclogenesis, suggesting that sand fly-derived promastigotes contain a substantial number of metacyclics, and/or that their differentiation status as metacyclics is more advanced in these populations. Infection experiments and studies evaluating the proportion of metacyclic promastigotes in culture and within the sand fly gut, previously reported by us, support the last hypothesis.es_ES
dc.description.sponsorshipThis work was funded by the Ramón Areces Foundation (contract 050204100014, OTT code 20100338) and by the network Red de Investigación Cooperativa en Enfermedades Tropicales.es_ES
dc.subjectLeishmania infantumes_ES
dc.subjectPhlebotomus perniciosuses_ES
dc.subjectAxenic culturees_ES
dc.titleRNA-seq analysis reveals differences in transcript abundance between cultured and sand fly-derived Leishmania infantum promastigoteses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderFundación Ramón Areceses_ES
dc.contributor.funderRed de Investigación Cooperativa en Enfermedades Tropicales (España)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidAlcolea, Pedro J. [0000-0002-0729-8941]es_ES
dc.contributor.orcidAlonso, Ana [0000-0002-1228-7331]es_ES
dc.contributor.orcidPaisie, Carolyn [0000-0003-4306-4154]es_ES
dc.contributor.orcidRamasamy, G. [0000-0001-7497-2919]es_ES
dc.contributor.orcidJiménez, Maribel [0000-0002-5615-3087]es_ES
dc.contributor.orcidMolina, Ricardo 0000-0001-6662-173X]es_ES
dc.contributor.orcidLarraga, Vicente [0000-0003-1260-7400]es_ES
dc.contributor.orcidMyler, P. J. [0000-0002-0056-0513 ]es_ES
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