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Title

Cytotoxicity of copper oxide nanoparticles on haemocytes of the marine bivalve Ruditapes philippinarum: an in vitro approach

AuthorsVolland, Moritz; Hampel, Miriam ; Katsumiti, Alberto; Yeste, María Pilar; Gatica, José Manuel; Cajaraville, Miren; Blasco, Julián
Issue DateSep-2016
Citation30th ESCPB Congress (2016)
AbstractCopper oxide nanoparticles (CuO NPs) are increasingly investigated, developed and manufactured for a wide and promising array of commercial and industrial products. Their increased production use and disposal will eventually lead to their increased release into the aquatic environment and potentially affect non-target organisms within. Although CuO NPs have been identified to be potentially cytotoxic, little is known about their behaviour in the marine environment and its potential influence on their cytotoxicity. We studied aggregation and dissolution kinetics of two commercially available bare copper oxide nanoparticles with nominal similar mean sizes (~ 40 nm), but distinct synthesis processes (wet chemistry and combustion synthesis) in various environmental and experimental relevant conditions. In addition cytotoxicity and DNA damage, as well as gene expression of oxidative stress, inflammatory response, DNA damage repair and cell death mediator markers were studied in Ruditapes philippinarum haemocytes after 24 h in vitro exposure and compared with sub-micron CuO (~ 500 nm) and water soluble Cu treated cells. Our results indicate that aggregating behaviour largely influences the toxicity of CuO NPs by influencing their susceptibility to ion leaching from the particle/aggregate surface. Gene expression analysis identified highly similar modes of action for all tested particulate and ionic Cu forms, further substantiating that nano and bulk CuO toxicity might largely be driven by ionic Cu. In addition our work highlights various differences in the behaviour of CuO NPs in environmental and culture conditions that need consideration when extrapolating results from in vitro to natural (or environmental) conditions.
DescriptionTrabajo presentado en el 30th ESCPB Congress (New European Society for Comparative Physiology and Biochemistry, "Unraveling complexity: from molecules to ecosystems"), celebrado en Barcelona del 4 al 7 de septiembre de 2016.
URIhttp://hdl.handle.net/10261/175480
Appears in Collections:(ICMAN) Comunicaciones congresos
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