Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/175154
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse the induced insulin resistance in pancreatic cells |
Autor: | Lima Cabello, Elena; Morales-Santana, Sonia; León, J.; Alché Ramírez, Víctor; Clemente, Alfonso CSIC ORCID; Alché Ramírez, Juan de Dios CSIC ORCID; Jiménez-López, José Carlos CSIC ORCID | Fecha de publicación: | 2018 | Editor: | Royal Society of Chemistry (UK) | Citación: | Food & function 9: 5176- 5188 (2018) | Resumen: | Insulin resistance (IR) is the main contributor to the development of type 2 diabetes. In this study, we have purified recombinant β-conglutin proteins (rβ1 to rβ4, and rβ6) from narrow-leafed lupin (NLL) by using affinity chromatography. The objective of this study was to evaluate the capacity of these β-conglutins to improve the IR state using ex vivo and in vitro systems. rβ1, rβ3, and rβ6 produced lower levels of pro-inflammatory mediator nitric oxide (about -7-fold in all cases), up-regulated mRNA expression levels of IRS-1 (+201, +173, +192%) and Glut-4 (+286, +121, +147%), increased levels of p85-PI3K (+188, +187, +137-fold) and Glut-4 (+503, +548, +515-fold) proteins, higher phosphorylation levels of the insulin signalling pathway activator p-IRS-1 and downstream mediators such as p-Akt, p-Cbl, and p-caveolin, and improved glucose uptake in insulin resistant (IR-C) culture cells. β-conglutin proteins were able to suppress the oxidative stress produced by insulin-induced resistance on PANC-1 control (C) cells by strongly reducing the protein oxidative carbonylation induced by ROS and balancing the metabolic homeostasis in IR-C cells through regulation of mRNA expression. At the same time, β-conglutins are able to reduce the levels of the pro-inflammatory mediator nitric oxide and promote the anti-oxidative capacity of cells by increasing the levels of reduced glutathione. These results suggest NLL β-conglutins might play a fundamental role as functional food components, since β-conglutins' nutraceutical properties could enhance the effectiveness of dietary improvement of type 2 diabetes complications. | URI: | http://hdl.handle.net/10261/175154 | DOI: | 10.1039/c8fo01164h | Identificadores: | doi: 10.1039/c8fo01164h issn: 2042-650X |
Aparece en las colecciones: | (EEZ) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2018_LimaCabello_FF pre.pdf | 1,41 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
8
checked on 22-mar-2024
WEB OF SCIENCETM
Citations
5
checked on 25-feb-2024
Page view(s)
201
checked on 28-mar-2024
Download(s)
174
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.