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Title

A membranar subproteome approach to understand the relationship between the polychaete Branchipolynoe seepensis and the mussel Bathymodiolys azoricus from Lucky Strike hydrothermal vent field

AuthorsCardoso, Cátia; Gomes, Tania; Osório, H.; Blasco, Julián ; Colaço, Ana; Santos, Ricardo Serrão; Bebianno, Maria João
Issue DateAug-2015
Citation14th Deep-Sea Biology Symposium (2015)
AbstractLucky Strike is the biggest hydrothermal vent field located in the Mid-Atlantic Ridge (MAR) at 1, 700m de pth, characterized by fluid chemistry with high metal concentrations (e.g. Cu, Fe, Mn and Zn), low pH and temperatures of 330ºC. The polychaete Branchipolynoe seepensis live in the palleal cavity of the mussel dominant species Bathymodiolus azoricus. B.seepensis is known by clean the mussel gills surface from particles in excess and its presence is above 85% in Lucky Strike vent field. The aim of this study was to understand, at a membranar subproteome level, if the presence of B.seepensis in vent mussel B.azoricus is a response to high metal concentrations in Lucky Strike vent sites (Montsegur-MS and Eiffel Tower-ET). Mussels and polychaetes were collected from Lucky Strike hydrothermal vent field (MS and ET), in August 2013, aboard the vessel “Pourquoi Pas?”. Subcellular fractionation of polychaetes and mussel gills was performed and 2D-SDS-PAGE applied to the membranar subproteome. Metal concentrations (e.g. Ag, Cd, Cu, Fe and Zn) of both species were also determined in cellular and subcellular fractions by ICP-OES. Protein expression signatures showed significant differences, suggesting intra variation in Lucky Strike field. For B. seepensis 471 different proteins were detected between the two vent sites, 11 specific for MS and 382 specific for ET and 71 and 67 specific proteins for B.azoricus for MS and ET, respectively, out of a total of 176. Common proteins to both sites with the higher change in expression (2-fold or higher) were identified by MALDI-TOF. Metal concentrations in the cellular (insoluble) and subcellular (heat-stable compounds) fractions were higher for both species in ET and MS, respectively. Data suggests that the different metal concentrations found in these species from both sites reflect the environmental chemical fluctuation between the two microhabitats within the same vent field and are related with the changes at the subproteome level. For B.seepensis protein changes are clear, suggesting that polychaetes from ET contain specific proteins that help to deal with excess of metals, regulating the metal accumulation in themussel gills.
DescriptionTrabajo presentado en el 14th Deep-Sea Biology Symposium, celebrado en Aveiro del 31 de agosto al 4 de septiembre de 2015.
URIhttp://hdl.handle.net/10261/175106
Appears in Collections:(ICMAN) Comunicaciones congresos
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