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Título: | Estudio de la fracción de carbohidratos en fórmulas infantiles |
Autor: | Sabater, Carlos CSIC ORCID | Director: | Montilla, Antonia CSIC ORCID; Prodanov, M. CSIC ORCID | Fecha de publicación: | 2014 | Resumen: | Human milk oligosaccharides have shown to have a wide range of biological activities including their prebiotic effect. Since breastfeeding is not always possible, infant formulae are supplemented with prebiotic oligosaccharides. Numerous studies point out the benefits of mixtures of galacto-oligosaccharides (GOS) and fructo-oligosaccharides, (FOS) ratio 9/1 to exert similar effects to breastfeed on children Nowadays, a great number of infant formula with prebiotics are disposal in market, however not data have been reported on their composition. Therefore, the determination of mono-, di- and oligosaccharide content of different commercial infant formulas, special infant formula and dairy drinks has been carried out. Initially a precipitation with Carrez as clarifying agent was selected for sample pre-treatment and two complementary chromatographic methods have been applied (GC-FID and HPLC-RID) for the separation and quantification of carbohydrates, showing their advantages, disadvantages and applications. According to the results obtained, GC-FID is a more adequate technique which allows a better qualitative and quantitative determination of carbohydrates of polymerization degree up to 7. Nevertheless, the GC-FID profiles allowed the identification of the specific GOS and FOS commercial mixture that was added during the formulas elaboration. For HPLC analysis, four ratio acetonitrile/water as mobile phase were tested, being 55:45 the best option. HPLC-RID analysis allowed the detection of polymers formed by 16 monomers. However, the individual quantification of single compounds was not possible.. The utilization of both techniques allows an overall characterization of the carbohydrate fraction, including prebiotic oligosaccharides in commercial infant formulae. | Descripción: | Trabajo Fin de Grado. | URI: | http://hdl.handle.net/10261/175045 |
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TFGCarbohidratosFórmulasInfantil.pdf | 1,4 MB | Adobe PDF | Visualizar/Abrir |
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