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Daily feeding behavior in Senegal sole larvae: improving feeding protocols

AuthorsNavarro-Guillén, Carmen; Moyano, Francisco Javier; Yúfera, Manuel
Issue DateMay-2014
Citation16th International Symposium on Fish Nutrition and Feeding (2014)
AbstractBoth the improvement of current feeding protocols of marine fish larvae based on live prey and the design of suitable inert feeds requires a good knowledge of the complexity of larval feeding behavior and of factors modulating food processing. Some of these factors affecting net efficiency of the digestive process are: the species, developmental stage, and diet composition. On the other hand, daily feeding ration and number of meals per day affect the absorption and assimilation of nutrients, and hence final growth. Senegalese sole (Solea senegalensis) has become a species with high commercial interest for aquaculture. The rapid physiological changes that Senegalese sole larvae undergo throughout development, including a pelagic pre-metamorphic and a benthic post-metamorphosis phase, complicate the determination of their feeding needs during larvae and post-larvae rearing. In this study, the daily feeding behavior and digestive enzymes activity in S. senegalensis were analyzed in pre-metamorphic (3 and 6 days post hatching; dph) and post-metamorphic larvae (20 and 30 dph) during a 24h cycle, with a photoperiod of 12 light:12 dark. Gut content was measured by counting rotifers mastax in pre-metamorphic larvae and by weighing the dissected gut in post-metamorphic larvae. Enzymes activity (trypsin and lipase) was analyzed individually per larvae using fluorogenic substrates. Results showed that Senegalese sole larvae have a diurnal feeding behavior during the pelagic phase, reaching the highest gut content at the end of the light period. On the other hand, metamorphosed larvae showed feeding activity during the whole daily cycle, although the gut content tended to be higher during nocturnal hours. Trypsin activity at pre-metamorphic stages was closely related to the amount of food present in the digestive tract while in post-metamorphic larvae the activity kept almost constant during the whole day, with the exception of first and last hours in the day, when activity levels were lower. Regarding to lipase activity, results point to a continuous production of lipase, although activity levels tended to be higher during dark phase in all stages. These results clearly confirm that moving from pelagic to benthic life involves a change in the processes related to feeding and digestion by sole post-larvae. Future experiments will explore whether this pattern may continue changing beyond the first month of life up to acquiring a definitive juvenile feeding strategy. This knowledge will allow the designof more appropriate feeding protocols during the first weeks for a better nutrient utilization and therefore enhanced growth and development.
DescriptionTrabajo presentado en eñ 16th International Symposium on Fish Nutrition and Feeding (ISFNF 2014), celebrado en Cairns (Australia) del 25 al 30 de mayo de 2014.
Appears in Collections:(ICMAN) Comunicaciones congresos
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