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Title

Antioxidant responses and bioaccumulation in clam Scrobicularia plana exposed to arsenate

AuthorsTrombini, Chiara ; Hampel, Miriam ; Rodríguez-Moro, G.; García-Barrera, Tamara; Gómez-Ariza, José Luis; Blasco, Julián
Issue DateJul-2015
Citation10th Iberian Congress on Environmental Contamination and Toxicology (2015)
7th Iberoamerican Congress on Environmental Contamination and Toxicology (2015)
AbstractArsenic is a well known toxicant released into the environment through different industrial processes and agricultural usage which environmental concentrations normally between 1 and 10 μg/L reaching milligram concentrations in polluted areas. In the aquatic environment arsenic exists in different chemical forms being arsenate (Asv) is the most common form under oxic conditions. In the present study marine clam Scrobicularia plana was used as a model organism to investigate oxidative stress and bioaccumulation related to Asv exposure. Clams were exposed to two concentrations of Asv (10 and 100 μgL-1) for 14 days and samples were collected after a 1, 7 and 14 days of exposure. Antioxidant biomarker including glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPX), aceticholinesterase (AchE) and superoxide dismutase (SOD) as well as lipid peroxidation (LPO) in gills and digestive glands were analyzed. .SOD activity increased in both tissues after one day of exposure to the highest concentration of Asv . A significant decrease in AchE and GR activities was observed in gills after seven days at both concentrations. Also GR activity decreased in digestive gland at the day 14. On the other hand, GST, GX and LPO didn’t show any significant alteration over time. The results indicate that exposure to environmental concentrations of Asv can induce oxidative stress. The concentration of Asv was determined in clam digestive gland: S. Plana showed a moderate bioaccumulation ability when exposed to both concentrations of Asv. .
DescriptionTrabajo presentado en el 10th Iberian Congress on Environmental Contamination and Toxicology y el 7th Iberoamerican Congress on Environmental Contamination and Toxicology (CICTA 2015), celebrados en Vila Real (Portugal) del 14 al 17 de julio de 2015.
URIhttp://hdl.handle.net/10261/174715
Appears in Collections:(ICMAN) Comunicaciones congresos
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