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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Segura, Joana | es_ES |
dc.contributor.author | Joshi, Ricky S. | es_ES |
dc.contributor.author | Díaz-Ingelmo, Ofelia | es_ES |
dc.contributor.author | Valdés, Antonio | es_ES |
dc.contributor.author | Dyson, Sílvia | es_ES |
dc.contributor.author | Martínez-García, Belén | es_ES |
dc.contributor.author | Roca, Joaquim | es_ES |
dc.date.accessioned | 2019-01-23T13:15:35Z | - |
dc.date.available | 2019-01-23T13:15:35Z | - |
dc.date.issued | 2018-09-28 | - |
dc.identifier.citation | Nature Communications 9: 3989 (2018) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/174607 | - |
dc.description.abstract | The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the “linking number paradox” of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns of DNA around the histone octamer and the DNA linking number difference (∆Lk) stabilized by individual nucleosomes, which has been experimentally estimated to be about −1.0. Here, we analyze the DNA topology of a library of mononucleosomes inserted into small circular minichromosomes to determine the average ∆Lk restrained by individual nucleosomes in vivo. Our results indicate that most nucleosomes stabilize about −1.26 units of ∆Lk. This value balances the twist (∆Tw ≈ + 0.2) and writhe (∆Wr ≈ −1.5) deformations of nucleosomal DNA in terms of the equation ∆Lk = ∆Tw + ∆Wr. Our finding reconciles the existing discrepancy between theoretical and observed measurement of the ΔLk constrained by nucleosomes. | es_ES |
dc.description.sponsorship | This work was supported by grants from Plan Estatal de Investigación Científica y Técnica of Spain to J.R. (BFU2015-67007-P and MDM-2014-0435-02). J.R. is a Professor at the Spanish National Research Council (CSIC). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-67007-P | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2014-0435-02 | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Intracellular nucleosomes constrain a DNA linking number difference of −1.26 that reconciles the Lk paradox | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1038/s41467-018-06547-w | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1038/s41467-018-06547-w | es_ES |
dc.identifier.e-issn | 2041-1723 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.contributor.orcid | Segura, Joana [0000-0002-2170-2701] | es_ES |
dc.contributor.orcid | Díaz-Ingelmo, Ofelia [0000-0002-9037-561X] | es_ES |
dc.contributor.orcid | Valdés, Antonio [0000-0002-9119-3092] | es_ES |
dc.contributor.orcid | Dyson, Sílvia [0000-0002-0126-3633] | es_ES |
dc.contributor.orcid | Roca, Joaquim [0000-0003-1462-954X] | es_ES |
dc.identifier.pmid | 30266901 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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