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Gas chromatographic-mass spectrometric analysis of galactosyl derivatives obtained by the action of two different β-galactosidases

AuthorsCardelle-Cobas, Alejandra ; Martínez Villaluenga, Cristina ; Sanz, M. Luz ; Montilla, Antonia
KeywordsPectinex Ultra SP-L
Lactozym 3000 L HP G
Issue Date2009
CitationFood Chemistry 114(3): 1099-1105 (2009)
AbstractDi- and trisaccharides obtained by transgalactosylation reaction of lactose using two different commercial enzyme preparations (Pectinex Ultra SP-L and Lactozym 3000 L HP G) have been characterised by gas chromatography-mass spectrometry as their trimethylsilyl oximes. Although mass fragments are common for most of the glycosidic linkages, their relative abundances can be used to determine carbohydrate identity. Moreover, formation of galactosyl- and digalactosyl-glycerols from the reaction mixture of Pectinex Ultra SP-L with lactose has been described by first time. Evolution of these compounds during transgalactosylation reactions and their influence on galactooligosaccharides (GOS) synthesis have been also evaluated.
Publisher version (URL)https://doi.org/10.1016/j.foodchem.2008.10.057
Identifiersdoi: 10.1016/j.foodchem.2008.10.057
issn: 0308-8146
Appears in Collections:(IQOG) Artículos
(IFI) Artículos
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