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dc.contributor.authorRos, Orioles_ES
dc.contributor.authorBarrecheguren, Pablo Josées_ES
dc.contributor.authorCotrufo, Tizianaes_ES
dc.contributor.authorSchaettin, Martinaes_ES
dc.contributor.authorRoselló-Busquets, Cristinaes_ES
dc.contributor.authorVílchez-Acosta, Albaes_ES
dc.contributor.authorHernaiz-Llorens, Marces_ES
dc.contributor.authorMartínez-Marmol, Ramónes_ES
dc.contributor.authorUlloa, Faustoes_ES
dc.contributor.authorStoeckli. Esther T.es_ES
dc.contributor.authorAraújo, Sofia J.es_ES
dc.contributor.authorSoriano, Eduardoes_ES
dc.identifier.citationPLoS Genetics 14(6): e1007432 (2018)es_ES
dc.description.abstractAxonal growth and guidance rely on correct growth cone responses to guidance cues. Unlike the signaling cascades that link axonal growth to cytoskeletal dynamics, little is known about the crosstalk mechanisms between guidance and membrane dynamics and turnover. Recent studies indicate that whereas axonal attraction requires exocytosis, chemorepulsion relies on endocytosis. Indeed, our own studies have shown that Netrin-1/Deleted in Colorectal Cancer (DCC) signaling triggers exocytosis through the SNARE Syntaxin-1 (STX1). However, limited in vivo evidence is available about the role of SNARE proteins in axonal guidance. To address this issue, here we systematically deleted SNARE genes in three species. We show that loss-of-function of STX1 results in pre- and post-commissural axonal guidance defects in the midline of fly, chick, and mouse embryos. Inactivation of VAMP2, Ti-VAMP, and SNAP25 led to additional abnormalities in axonal guidance. We also confirmed that STX1 loss-of-function results in reduced sensitivity of commissural axons to Slit-2 and Netrin-1. Finally, genetic interaction studies in Drosophila show that STX1 interacts with both the Netrin-1/DCC and Robo/Slit pathways. Our data provide evidence of an evolutionarily conserved role of STX1 and SNARE proteins in midline axonal guidance in vivo, by regulating both pre- and post-commissural guidance mechanismses_ES
dc.description.sponsorshipThis work was funded by MINECO (to ES: SAF2013-42445-R, SAF2016-7426 and BFU2010-21507; FU: RYC-2007-00417; and SJA: RYC-2009-05510), ISCIII (to ES: CIBERNED), Ministerio de Educación, Cultura y Arte (to OR: AP2005-1662), Fundació La Caixa (to PJB) and the Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (to ETS: 3100A_130730 and 31003A_166479).es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleA conserved role for Syntaxin-1 in pre- and post-commissural midline axonal guidance in fly, chick, and mousees_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)es_ES
dc.contributor.funderObra Social la Caixaes_ES
dc.contributor.funderSwiss National Science Foundationes_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidRos, Oriol [0000-0003-4589-9593]es_ES
dc.contributor.orcidCotrufo, Tiziana [0000-0002-1671-3051]es_ES
dc.contributor.orcidSoriano, Eduardo [0000-0001-9260-9291]es_ES
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