In vitro Simulation of protein digestive bioaccesibility in Senegalese sole using the response surface methodology

Abstract

Considering the digestive system as a bioreactor may give an insight on the relative influence of different factors affecting its functionality, helping to optimize it. The present work uses a novel approach to develop a mathematical model explaining the effect of three main factors - temperature, total reaction time, and enzyme:substrate ratio - in the hydrolysis of proteins by intestinal enzymes in the Senegalese sole (Solea senegalensis). We combined a factorial design, based on the Response Surface Methodology, and in vitro digestibility assays adapted to the physiology and culture conditions of this species. This fast, non-invasive, and low-cost methodology can be used as a tool to maximize the biological response under variable conditions and may orientate on-farm feeding practices.