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Avenues to characterize the interactions of extended N-Glycans with proteins by NMR spectroscopy: The influenza hemagglutinin case

AutorFernández de Toro, Beatriz; Peng, Wenjie; Thompson, Andrew J.; Domínguez, Gemma; Cañada, F. Javier ; Pérez-Castells, Javier; Paulson, James C.; Jiménez-Barbero, Jesús ; Canales, Ángeles
Palabras claveN-glycan
Paramagnetic NMR
Pseudo contact shifts
Sialic acid
Fecha de publicación12-nov-2018
CitaciónAngew Chem Int Ed 57:15051 –15055 (2018)
ResumenLong-chain multiantenna N-glycans are extremely complex molecules. Their inherent flexibility and the presence of repetitions of monosaccharide units in similar chemical environments hamper their full characterization by X-ray diffraction or standard NMR methods. Herein, the successful conformational and interaction analysis of a sialylated tetradecasaccharide N-glycan presenting two LacNAc repetitions at each arm is presented. This glycan has been identified as the receptor of the hemagglutinin protein of pathogenic influenza viruses. To accomplish this study, a N-glycan conjugated with a lanthanide binding tag has been synthesized, enabling analysis of the system by paramagnetic NMR. Under paramagnetic conditions, the NMR signals of each sugar unit in the glycan have been determined. Furthermore, a detailed binding epitope of the tetradecasaccharide N-glycan in the presence of HK/68 hemagglutinin is described. © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
Descripción5 p.-2 fig.1 sch. Dedicated to Prof. Manuel Martín-Lomas on the occasion of his 77th birthday
Versión del editorhttps://doi.org/10.1002/anie.201807162
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