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Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM

AutorBenaglia, Simone; Gisbert, Victor G.; Perrino, Alma P. ; Amo, Carlos A.; García García, Ricardo
Palabras claveApplications of AFM
Atomic force microscopy
Nanoscale biophysics
Fecha de publicación16-nov-2018
EditorSpringer Nature
CitaciónNature Protocols 13: 2890–2907 (2018)
Resumen[EN] Fast, high resolution and wide elastic modulus range mapping of heterogeneous interfaces represents a major goal of atomic force microscopy (AFM). This goal becomes more challenging when the nanomechanical mapping involves biomolecules in their native environment. Over the years, several AFM-based methods have been developed to address that goal. However, none of those methods combine sub-nanometer spatial resolution, quantitative accuracy, fast data acquisition speed, wide elastic modulus range and operation in physiological solutions. Here we present detailed protocols to generate high resolution maps of the elastic properties of biomolecules and polymers by using bimodal AFM. The method is fast because the elastic modulus, deformation and topography images are obtained simultaneously. The method is efficient because just a single data point per pixel is needed to generate the above images. In addition, by knowing the deformation, bimodal AFM enables to reconstruct the true topography of the surface.
Versión del editorhttps://doi.org/10.1038/s41596-018-0070-1
URIhttp://hdl.handle.net/10261/172908
ISSN1754-2189
E-ISSN1750-2799
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