English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/172832
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

The singular properties of photosynthetic cytochrome c550 from the diatom Phaeodactylum tricornutum suggest new alternative functions

AuthorsBernal-Bayard, Pilar; Álvarez, Consolación ; Calvo, Purificación; Roncel, Mercedes; Hervás, Manuel ; Navarro, José A.
Issue DateNov-2018
CitationPhysiologia Plantarum, (2018)
AbstractCytochrome c550 is an extrinsic component in the luminal side of photosystem II in cyanobacteria, as well as in eukaryotic algae from the red photosynthetic lineage including, among others, diatoms. We have established that cytochrome c550 from the diatom Phaeodactylum tricornutum can be obtained as a complete protein from the membrane fraction of the alga, although a C‐terminal truncated form is purified from the soluble fractions of this diatom as well as from other eukaryotic algae. Eukaryotic cytochromes c550 show distinctive electrostatic features as compared with cyanobacterial cytochrome c550. In addition, co‐immunoseparation and mass spectrometry experiments, as well as immunoelectron microscopy analyses, indicate that although cytochrome c550 from P. tricornutum is mainly located in the thylakoid domain of the chloroplast –where it interacts with photosystem II–, it can also be found in the chloroplast pyrenoid, related with proteins linked to the CO2 concentrating mechanism and assimilation. These results thus suggest new alternative functions of this heme protein in eukaryotes.
Publisher version (URL)https://doi.org/10.1111/ppl.12888
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
Bernal_etal_PhysPlant.pdf Embargoed until November 28, 20196,68 MBAdobe PDFThumbnail
View/Open    Request a copy
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.