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dc.contributor.authorMarcos-López, Mares_ES
dc.contributor.authorCalduch Giner, Josep A.es_ES
dc.contributor.authorMirimin, Lucaes_ES
dc.contributor.authorMacCarthy, Eugenees_ES
dc.contributor.authorRodger, Hamish D.es_ES
dc.contributor.authorO'Connor, Ianes_ES
dc.contributor.authorSitjà-Bobadilla, Ariadnaes_ES
dc.contributor.authorPérez Sánchez, Jaumees_ES
dc.contributor.authorPiazzon de Haro, María Carlaes_ES
dc.identifier.citationScientific Reportses_ES
dc.description.abstractAmoebic gill disease (AGD) is one of the main diseases affecting Atlantic salmon (Salmo salar L.) mariculture. Hallmarks of AGD are hyperplasia of the lamellar epithelium and increased production of gill mucus. This study investigated the expression of genes involved in mucus secretion, cell cycle regulation, immunity and oxidative stress in gills using a targeted 21-gene PCR array. Gill samples were obtained from experimental and natural Neoparamoeba perurans infections, and sampling points included progressive infection stages and post-freshwater treatment. Up-regulation of genes related to mucin secretion and cell proliferation, and down-regulation of pro-inflammatory and pro-apoptotic genes were associated with AGD severity, while partial restoration of the gill homeostasis was detected post-treatment. Mucins and Th2 cytokines accoun ted for most of the variability observed between groups highlighting their key role in AGD. Two mucins (muc5, muc18) showed differential regulation upon disease. Substantial up-regulation of the secreted muc5 was detected in clinical AGD, and the membrane bound muc18 showed an opposite pattern. Th2 cytokines, il4/13a and il4/13b2, were significantly upregulated from 2 days post-infection onwards, and changes were lesion-specific. Despite the differences between experimental and natural infections, both yielded comparable results that underline the importance of the studied genes in the respiratory organs of fish, and during AGD progression.es_ES
dc.description.sponsorshipIrish Research Council (Employment Based Postgraduate Programme Project ID EBPPG/2013/2)es_ES
dc.description.sponsorshipEuropean Commission under the TNA programme (project ID AE030036) within AQUAEXCEL2020 project (652831).es_ES
dc.description.sponsorshipCSIC PIE project no. 201740E013.es_ES
dc.publisherSpringer Naturees_ES
dc.subjectAmoebic gill diseasees_ES
dc.subjectAtlantic salmones_ES
dc.subjectInterleukin 4/13es_ES
dc.subjectGene expressiones_ES
dc.subjectNeoparamoeba peruranses_ES
dc.titleGene expression analysis of Atlantic salmon gills reveals mucin 5 and interleukin 4/13 as key molecules during amoebic gill diseasees_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderIrish Research Counciles_ES
dc.contributor.funderCSIC - Instituto de Acuicultura de Torre de la Sal (IATS)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidCalduch Giner, Josep A. [0000-0003-3124-5986]es_ES
dc.contributor.orcidSitjà-Bobadilla, Ariadna [0000-0002-7473-3413]es_ES
dc.contributor.orcidPérez Sánchez, Jaume [0000-0003-2506-1523]es_ES
dc.contributor.orcidPiazzon de Haro, María Carla [0000-0002-4949-8984]es_ES
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