English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/172629
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

Gene expression analysis of Atlantic salmon gills reveals mucin 5 and interleukin 4/13 as key molecules during amoebic gill disease

AutorMarcos-López, Mar; Calduch Giner, Josep A.; Mirimin, Luca; MacCarthy, Eugene; Rodger, Hamish D.; O'Connor, Ian; Sitjà-Bobadilla, Ariadna ; Pérez Sánchez, Jaume; Piazzon de Haro, María Carla
Palabras claveAmoebic gill disease
Atlantic salmon
Mucins
Interleukin 4/13
Gene expression
Neoparamoeba perurans
Fecha de publicación12-sep-2018
EditorSpringer Nature
CitaciónScientific Reports
ResumenAmoebic gill disease (AGD) is one of the main diseases affecting Atlantic salmon (Salmo salar L.) mariculture. Hallmarks of AGD are hyperplasia of the lamellar epithelium and increased production of gill mucus. This study investigated the expression of genes involved in mucus secretion, cell cycle regulation, immunity and oxidative stress in gills using a targeted 21-gene PCR array. Gill samples were obtained from experimental and natural Neoparamoeba perurans infections, and sampling points included progressive infection stages and post-freshwater treatment. Up-regulation of genes related to mucin secretion and cell proliferation, and down-regulation of pro-inflammatory and pro-apoptotic genes were associated with AGD severity, while partial restoration of the gill homeostasis was detected post-treatment. Mucins and Th2 cytokines accoun ted for most of the variability observed between groups highlighting their key role in AGD. Two mucins (muc5, muc18) showed differential regulation upon disease. Substantial up-regulation of the secreted muc5 was detected in clinical AGD, and the membrane bound muc18 showed an opposite pattern. Th2 cytokines, il4/13a and il4/13b2, were significantly upregulated from 2 days post-infection onwards, and changes were lesion-specific. Despite the differences between experimental and natural infections, both yielded comparable results that underline the importance of the studied genes in the respiratory organs of fish, and during AGD progression.
Versión del editorhttp://dx.doi.org/10.1038/s41598-018-32019-8
URIhttp://hdl.handle.net/10261/172629
DOI10.1038/s41598-018-32019-8
Aparece en las colecciones: (IATS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
s41598-018-32019-8.pdf2,8 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.